Postnatal brain maturation (WP2)
Define the role of serotonin in postnatal maturation of the raphe-PFC pathway.
We will define how perinatal developmental changes in the raphe-PFC pathway as studied in WP1 affect PFC postnatal brain maturation, behaviour and cognition across (pre) adolescence and adulthood. We will also study how these processes are affected by sex, stress and adult SSRI exposure. To these ends, WP2 makes use of transgenic
rodent models, state-of-the-art technologies such as in utero electroporation, fiber photometry, optogenetics, electrophysiology, MRI, and touchscreen-based cognitive testing (refined in WP4), and applies computational analyses to model the effect of early changes in serotonin signaling on postnatal brain maturation and behaviour.